Development of an Aptamer-based Lateral Flow Device for Point-of-care Detection of Toxic C
Aconitine and related alkaloids are potent cardiotoxins and neurotoxins found in the Aconitum species used in traditional Chinese medicine (TCM), such as 'Chuanwu', 'Caowu' and 'Fuzi'. They pose significant health risks when used inappropriately without professional supervision. Patients with acute aconite poisoning of
| Condition(s) | Aconite Poisoning |
|---|---|
| Status | Recruiting |
| Study type | Observational |
| Summary | Aconitine and related alkaloids are potent cardiotoxins and neurotoxins found in the Aconitum species used in traditional Chinese medicine (TCM), such as 'Chuanwu', 'Caowu' and 'Fuzi'. They pose significant health risks when used inappropriately without professional supervision. Patients with acute aconite poisoning often present with a combination of cardiovascular, neurological, and gastrointestinal toxicities, which can occur after an overdose, inadequate processing of the aconite roots, erroneous use of tincture preparations, and contamination or substitution of other herbs with aconite roots. Timely diagnosis of aconite poisoning remains challenging due to the long laboratory turnaround time. The aim of this study is to develop an aptamer-based lateral flow device (LFD) for point-of-c |
| Who can participate | Inclusion Criteria (Aconite Poisoned Group): * adult patients aged 18 years or above * clinical presentations consistent with acute aconite poisoning, including neurological, gastrointestinal, or cardiovascular symptoms * exposure to aconite alkaloids within 3 days Exclusion Criteria (Aconite Poisoned Group): * refusal of informed consent for urine collection * alternative explanations for clinical presentations Inclusion Criteria (Aconite Therapeutic Use Group): * adult patients aged 18 years or above * documented history of using aconite for therapeutic purposes, as prescribed by a qualified practitioner in a Chinese medicine clinic * exposure to aconite alkaloids within 3 days Exclusion Criteria (Aconite Therapeutic Use Group): \- Refusal of informed consent for urine collection. Inclus |
| Ages | 18 Years |
| Sex | All |
| Accepts healthy volunteers | Yes |
| Lead sponsor | The University of Hong Kong |
| Locations | Hong Kong, Hong Kong; Hong Kong, Hong Kong |
| Start date | 2025-10-15 |
| NCT ID | NCT07456592 |
| Official listing | https://clinicaltrials.gov/study/NCT07456592 |