Exhaled Breath Condensate Analysis in Mechanically Ventilated Patients
Mechanically ventilated intensive care patients will be sampled for a small amount of exhaled breath condensate from the ventilator circuit and for venous blood. Proteomic analysis of the exhaled breath condensate will be performed using mass spectrometry and in the blood sample, corresponding changes in the DNA, RNA,
| Condition(s) | Acute Respiratory Failure, Critical Illness |
|---|---|
| Status | Recruiting |
| Study type | Observational |
| Summary | Mechanically ventilated intensive care patients will be sampled for a small amount of exhaled breath condensate from the ventilator circuit and for venous blood. Proteomic analysis of the exhaled breath condensate will be performed using mass spectrometry and in the blood sample, corresponding changes in the DNA, RNA, proteins, and metabolites will be studied. Resulting profiles will be correlated with routinely monitored parameters in order to identify patterns corresponding to various pathologies in order to enable their early detection. |
| Who can participate | Inclusion Criteria: Intensive care setting Mechanical ventilation Ventilator/ventilator circuit technically suitable for sampling of exhaled breath condensate Exclusion Criteria: Mechanical ventilation challenging to the extent that the insertion of the sampling kit into the ventilator circuit would substantially increase the risk for respiratory deterioration |
| Ages | 18 Years |
| Sex | All |
| Lead sponsor | The Institute of Molecular and Translational Medicine, Czech Republic |
| Locations | Olomouc, Czechia; Olomouc, Czechia |
| Start date | 2026-03-01 |
| NCT ID | NCT06189924 |
| Official listing | https://clinicaltrials.gov/study/NCT06189924 |